Nano Cell Culture
CELL CULTURE DEVICE
Inventors: Yong Chen, Jian Shi, Li Wang, Yadong Tang
Laboratory: Processus d'Activation Sélectif par Transfert d'Energie Uni-électronique ou Radiatif (PASTEUR - UMR8640).
Technical domain: Life science, molecular biology, and clinical diagnostic.
Intellectual property: PCT/EP2016/066366
Crosslinked monolayer nanofibers with mesh size comparable to that of mammalian cells are used for off-ground culture and differentiation of pluripotent stem cells. Unlike conventional methods, cells on such a device are subjected to limited exogenous contact but optimal permeability, allowing controlled differentiation of pluripotent stem cells and formation of cell patches of different cell types.
The cell patches can be used for both in vitro and in vitro assays. They can also be integrated into a microfluidic device to form body mimics or be circulated under physiological conditions, which should be important for next generation biomedical research, drug test, disease modeling, regenerative medicine, etc.